HNRPM (HNRNPM) Mouse Monoclonal Antibody [Clone ID: 1D8]

Specifications

Product Data
Clone Name	1D8
Applications	ICC/IF, IHC, IP, WB
Recommend Dilution	Immunohistochemistry-Paraffin: 1:200, Immunohistochemistry: 1:200 - 1:500, Western Blot: 1:500, Immunohistochemistry-Frozen: 1:10-1:500, Immunocytochemistry/ Immunofluorescence: 1:10-1:500, Immunoprecipitation: 1:10-1:500
Reactivity	Human, Mouse, Rat, Bovine, Porcine, Rabbit
Host	Mouse
Clonality	Monoclonal
Immunogen	M19 fusion protein containing full-length human protein. [UniProt# P52272]
Formulation	Preservative: 0.05% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Ascites
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	heterogeneous nuclear ribonucleoprotein M
Database Link	NP_112480 Entrez Gene 76936 MouseEntrez Gene 116655 RatEntrez Gene 4670 Human UniProt ID P52272
Background	RNA-binding proteins of mammalian hnRNP (heterogeneous nuclear ribonucleo protein) family represents a group of over 20 distinct evolutionary conserved proteins which share a capability to bind pre-mRNA/mRNA, and also binds to specific RNA motifs with high-affinity. They exhibit huge functional diversity and implicates in almost every step in mRNA's life cycle (from transcription and processing to transport and translation). HnRNP M is an abundant protein containing three RNA recognition motifs (RRM), a glycine/methionine-rich region and a methionine/arginine-rich repeat motif. It has four protein variants, hnRNP M1-4, ranging in molecular size from 68 to 72 kD which corresponds to spliced isoforms and/or posttranslationally modified forms. hnRNP M is a nucleolar protein and has been Identified in the spliceosome C complex. It acts as a pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro, and is involved in splicing. hnRNP M acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and TNF-alpha cytokines.
Synonyms	CEAR; hnRNP M; HNRNPM4; HNRPM; HNRPM4; HTGR1; NAGR1
Note	This hnRNP M1-M4 (1D8) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded and frozen sections, Immunoprecipitation and Western blot. A 64-68 kDa band can be seen in a Western blot using HeLa cell lysate.
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Spliceosome

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