p16INK4A (CDKN2A) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FC, ICC/IF, IHC, IP, WB
Recommend Dilution	Flow Cytometry: 1:400, ELISA: 1:100-1:2000, Immunohistochemistry-Paraffin: 1:10-1:500, Knockdown Validated, Immunohistochemistry: 1:10-1:500, Immunoprecipitation, Immunohistochemistry-Frozen: 1:10-1:500, Immunocytochemistry/ Immunofluorescence, Western Blot
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to a portion of human p14ARF (between residues 50-150). [Swiss-Prot# Q8N726]
Formulation	PBS, 30% glycerol, 0.1% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	cyclin-dependent kinase inhibitor 2A
Database Link	NP_000068 Entrez Gene 12578 MouseEntrez Gene 1029 Human UniProt ID Q8N726
Background	The INK4a-ARF locus is comprised of two tumor suppressors, p16INK4a and p14ARF. These two proteins are encoded through differential splicing of alternative first exons. The p16INK4a (exon 1 alpha) protein inhibits the cyclin D-dependent kinases (CDK) that control the phosphorylation of the Rb protein and cell proliferation. The p14ARF gene product complexes with the MDM2 protein within the nucleus, thus modulating the activity of the p53 protein. P14ARF is a potent tumor suppressor in the presence of wild-type p53, while mutant p53 substantially reduces growth inhibition by p14ARF.
Synonyms	ARF; CDK4I; CDKN2; CMM2; INK4; INK4A; MLM; MTS-1; MTS1; P14; P14ARF; P16; P16-INK4A; P16INK4
Note	This p14ARF antibody is used for Western blotting, where a band is seen at where a band is seen at ~16 kDa, representing p14ARF (p14ARF tends to run slightly higher than the theoretical MW of 14 kDa). Additional faint bands may be seen at ~32 and 47 kDa. WB application of this product is mentioned in publication with PMID: 21636682. In ICC/IF, nuclear focal staining was observed in HeLa cells.
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Bladder cancer, Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer

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