BTK Mouse Monoclonal Antibody [Clone ID: 7F12H4]

Specifications

Product Data
Clone Name	7F12H4
Applications	ICC/IF, IHC, WB
Recommend Dilution	Immunohistochemistry: 1:10-1:500, Immunocytochemistry/ Immunofluorescence: 1:200-1:1000, Western Blot: 1:500-1:2000, Immunohistochemistry-Paraffin: 1:200-1:1000
Reactivity	Human, Primate
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of BTK expressed in E. coli. [UniProt# Q06187]
Formulation	PBS, 0.03% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Ammonium sulfate precipitation
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	77 kDa
Gene Name	Bruton tyrosine kinase
Database Link	NP_000052 Entrez Gene 695 Human UniProt ID Q06187
Background	BTK, also known as Brutons tyrosine kinase, is a member of the BTK/Tec family of cytoplasmic tyrosine kinases. All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini.BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation.
Synonyms	AGMX1; AT; ATK; BPK; IMD1; PSCTK1; XLA
Note	This BTK (7F12H4) antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA.
Reference Data
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	B cell receptor signaling pathway, Fc epsilon RI signaling pathway, Primary immunodeficiency

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