Apolipoprotein E (APOE) Mouse Monoclonal Antibody [Clone ID: WUE-4]

Specifications

Product Data
Clone Name	WUE-4
Applications	CyTOF-ready, ELISA, FC, ICC/IF, IHC, IP, WB
Recommend Dilution	Flow (Intracellular), Immunohistochemistry-Paraffin, Flow Cytometry: 1 ug per million cells, ELISA: 1:100-1:2000, Immunohistochemistry: 1:50-1:200, Immunoprecipitation: 1:10-1:500, Western Blot: 2 ug/ml, Immunocytochemistry/ Immunofluorescence: 1:200, CyTOF-ready
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified human ApoE [UniProt# P02649]
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	36 kDa
Gene Name	apolipoprotein E
Database Link	NP_000032 Entrez Gene 11816 MouseEntrez Gene 25728 RatEntrez Gene 348 Human UniProt ID P02649
Background	ApoE (apolipoprotein E) is the protein constituent of cholesterol/triglyceride-rich plasma lipoproteins, and is a multifunctional glycosylated secretary protein found almost in all organs with high activity in hepatic tissues. ApoE expression is induced by cholesterol-rich diets and is enhanced in lipoproteins in humans with genetic disorder type III hyperlipoproteinemia (HLP) characterized by remnant lipoproteins accumulation in plasma and premature atherosclerosis. ApoE circulates in blood as a protein component of VLDLs, chylomicron remnants, a subclass of HDL etc, and in cerebrospinal fluid as well as CNS interstitial fluid on small particles and disks resembling HDLs. ApoE facilitates transport of cholesterol and other lipids, as well as the clearance of plasma lipoproteins by serving as a critical ligand for lipoprotein uptake by LDL receptors and related proteins. ApoE participates in lipids redistribution to cells (e.g. CNS) that require cholesterol and phospholipids for reparative processes. ApoE also involves in proliferation inhibition of smooth muscle cells/lymphocytes, antigen presentation, and cholesterol efflux stimulation from foam cell macrophages. Defects in APOE have been linked to HLPP3 (hyperlipoproteinemia type 3), AD2 (Alzheimer disease type 2), SBHD (sea-blue histiocyte disease), LPG (lipoprotein glomerulopathy), and certain autoimmune disorders including multiple sclerosis and psoriasis.
Synonyms	AD2; APO-E; LDLCQ5; LPG
Note	This ApoE antibody is useful for Western blot, ELISA, Immunohistochemistry and Immunoprecipitation. In Western blot a band is observed at ~36 kDa, representing the ApoE protein.
Reference Data
Protein Families	Adult stem cells, Druggable Genome, Secreted Protein, Stem cell - Pluripotency
Protein Pathways	Alzheimer's disease

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