TOMM70A (TOMM70) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, Simple Western, WB
Recommend Dilution	Immunocytochemistry/ Immunofluorescence: 1:100, Western Blot: 1:500, Simple Western: 1:50
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to a region within residues 1-100 of the human TOM70 protein. [Swiss-Prot# O94826]
Formulation	PBS. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein A purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	67 kDa
Gene Name	translocase of outer mitochondrial membrane 70
Database Link	NP_055635 Entrez Gene 28185 MouseEntrez Gene 304017 RatEntrez Gene 9868 Human UniProt ID O94826
Background	Most of mitochondrial proteins are synthesized by cytosolic ribosomes followed by import into mitochondria post-translationally with the help of cytosolic chaperones as well as mitochondrial translocators (import components). Translocase of the outer membrane (TOM) complex (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70) is the proteinaceous machinery that contains surface receptors for pre-protein recognition and serve as main entry gateway into mitochondria. Mitochondrial targeting requires different cytosolic factors, in particular the molecular chaperones (Hsc70/Hsp70 and Hsp90) whose activity occur in coordinated cycles of ATP hydrolysis/substrate binding and are regulated by a number of co-chaperone proteins including TOM70, a member of TPR (tetratricopeptide repeat) co-chaperone family. TOM70 contains a conserved TPR clamp domain for interaction with Hsc70 and Hsp90, and such interaction is essential for import process initiation. TOMM70 is a single-pass membrane protein localized in mitochondrion outer membrane and is ubiquitously expressed in various tissues where it acts as a receptor that accelerates the import of all mitochondrial precursor proteins. In addition to importing inner membrane carrier proteins, Tom70 is involved in targeting Mcl-1 to outer membrane via an internal EELD sequence.
Synonyms	Tom70; TOMM70A
Note	This TOM70 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~67 kDa. In ICC/IF mitochondrial staining was observed in MCF7 cells.
Reference Data
Protein Families	Transmembrane

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