CXCR4 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, ICC/IF, IHC, WB
Recommend Dilution	Flow Cytometry, Immunocytochemistry/ Immunofluorescence: 1:50, Immunohistochemistry-Paraffin: 1:200-1:1000, Immunohistochemistry: 1:200-1:1000, Western Blot: 1:5000, Immunohistochemistry-Frozen
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to a C-terminal region of the human CXCR4 protein (within residues 300-352). [Swiss-Prot P61073]
Formulation	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Whole antisera
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	C-X-C motif chemokine receptor 4
Database Link	NP_003458 Entrez Gene 12767 MouseEntrez Gene 60628 RatEntrez Gene 7852 Human UniProt ID P61073
Background	CXCR4 (C-X-C chemokine receptor type 4) is a member of GPCR1 family and is expressed as a multipass membrane protein in several tissues wherein it acts as a receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. CXCR4 is involved in hematopoiesis, cardiac ventricular septum formation and plays an essential role in vascularization of the gastrointestinal tract via regulating vascular branching and/or remodeling processes in endothelial cells. CXCR4 is involved in cerebellar development and mediate hippocampal-neuron survival in CNS. It also acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates, and promotes Env-mediated fusion of the virus.
Synonyms	CD184; D2S201E; FB22; HM89; HSY3RR; LAP-3; LAP3; LCR1; LESTR; NPY3R; NPYR; NPYRL; NPYY3R; WHIM
Note	This CXCR4 antibody is useful for Immunohistochemistry-paraffin embedded sections, Immunocytochemistry/Immunofluorescence and Western Blot. In Western blot a band is observed ~ 40 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, GPCR, Transmembrane
Protein Pathways	Axon guidance, Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Endocytosis, Leukocyte transendothelial migration

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