beta Actin (ACTB) Rabbit Polyclonal Antibody

CAT#: TA336477

Rabbit Polyclonal beta-Actin Antibody



Need it in bulk or conjugated?
Get a free quote

CNY 5,381.00


货期*
5周

规格
    • 100 ug

Product images

经常一起买 (4)
Transient overexpression lysate of actin, beta (ACTB)
    • 100 ug

CNY 3,080.00


beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control
    • 30 ul

CNY 300.00
CNY 1,430.00


Recombinant protein of human actin, beta (ACTB), 20 µg
    • 20 ug

CNY 2,900.00
CNY 6,650.00


Recombinant protein of human actin, beta (ACTB), 100 µg
    • 100 ug

CNY 9,998.00

Specifications

Product Data
Applications ICC/IF, IHC, Simple Western, WB
Recommend Dilution Immunocytochemistry/ Immunofluorescence: 1:100, Simple Western: 1:12.5, Western Blot: 0.25-1 ug/ml, Immunohistochemistry-Paraffin: 10 ug/mL, Immunohistochemistry, Knockdown Validated
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Immunogen Amino acids 2-16 (CDDDIAALVIDNGSG) of actin protein were used as the immunogen.
Formulation PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration lot specific
Purification Protein G purified
Conjugation Unconjugated
Storage Condition Store at -20°C as received.
Gene Name actin, beta
Background Actin is a globular protein found in all eukaryotic cells (the only known exception being nematode sperm) where it may be present at concentrations of over 100 uM. It is the monomeric subunit of two types of filaments: microfilaments, one of the three major components of the cytoskeleton, and thin filaments, part of the contractile apparatus in muscle cells. Actin participates in many important cellular processes including muscle contraction, cell signaling, motility and division, cytokinesis, vesicle and organelle movement, and the establishment and maintenance of cell junctions and shape. In vertebrates, three main groups of actin isoforms, alpha, beta, and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cells as components of the cytoskeleton, and as mediators of internal cell motility (Doherty GJ, et al 2008). Among these actins, beta actin, which is expressed constantly, and at high levels, in all the tissue types used in protein research except muscle (skeletal and cardiac). Beta actin acts as a useful loading control in Western blot analysis. Loading controls serve a number of purposes; they ensure the protein of interest has been correctly loaded on the gel, that it is being transferred correctly, and that all reagents are functioning normally. Loading controls can also be used to compare amounts of protein tested (or loaded) per gel lane in western blotting. While very useful when comparing lysates of the same origin (for example transfected and untransfected HEK 293s), comparing lysates from different tissues can be complex. Actin is almost universally expressed, but different tissues express different amounts. In western blotting, an actin antibody titered to the detection limit can show these differences, while an actin antibody used well above the detection limit can show a response that appears equally robust in all samples probed. Therefore caution must be used when using actin or another 'loading control antibody.'
Synonyms BRWS1; PS1TP5BP1
Note This antibody is not suitable for testing heart or muscle lysate loading.
Reference Data
Protein Families ES Cell Differentiation/IPS
Protein Pathways Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Tight junction, Vibrio cholerae infection, Viral myocarditis
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...