CIKS (TRAF3IP2) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, IP, Simple Western, WB
Recommend Dilution	Immunohistochemistry, Western Blot: 1-2 ug/ml, Immunohistochemistry-Paraffin, Simple Western: 1:20, Immunoprecipitation
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	This antibody was developed against a synthetic peptide (QDLPRPLRSREFPQFEP) corresponding to amino acids 225-241 of human TRAF3IP2.
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	TRAF3 interacting protein 2
Database Link	NP_679211 Entrez Gene 103213 MouseEntrez Gene 10758 Human UniProt ID O43734
Background	NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines, such as IL-1 results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes (2). Recently, Li et al. (3) have isolated a cDNA coding for a protein named Act1 (NF- B activator 1). Act1/CIKS cDNA codes for a protein containing 574 aa with a predicated molecular mass of 60 kDa. Act1/CIKS protein contains a helix-loop-helix domain in its N-terminal portion and a coiled-coil structure at the C terminus. Act1 lacks any catalytic domain, however, it activates IKK through the helix-loop-helix domain. The activation of NF- B by Act1 appears to be through the NIK-IKK complex, because a NIK dominant-negative mutant protein inhibited Act1-induced NF- B activation. IL-1 responsive pathway is also necessary for Act1 function. Act1 mRNA is expressed in a variety of tissues, with high level in thymus, kidney and placenta; moderate level in heart, skeletal muscle, colon, liver, lung and small intestine; and at a very low level in brain, spleen, and peripheral blood leukocytes.
Synonyms	ACT1; C6orf2; C6orf4; C6orf5; C6orf6; CANDF8; CIKS; PSORS13
Reference Data

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