Langerin (CD207) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, IHC, WB
Recommend Dilution	Western Blot: 1 - 3 ug/ml, Flow Cytometry: 1:20-1:2000, Immunohistochemistry: 1:200-1:1000, Immunohistochemistry-Frozen: 1:200-1:1000, Immunohistochemistry-Paraffin: 1:200-1:1000, Flow (Intracellular)
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Amino acids 26-44 of mouse Langerin were used as immunogen. (Genbank Accession No. CAC82936)
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	CD207 molecule
Database Link	NP_056532 Entrez Gene 50489 Human UniProt ID Q9UJ71
Background	Langerin (CD207) is a type II membrane-associated C-type lectin known to be expressed exclusively by Langerhans cells. Langerin recognizes mannose residues via its single carbohydrate recognition domain (CRD). Langerin is localized not only on the cell surface, but also intracellularly in close association with Birbeck granules. Transfection of Langerin cDNA into fibroblasts creates a compact network of membrane structures with typical features of Birbeck granules (BG). Langerin is thus a potent inducer of membrane superimposition and zippering leading to BG formation.
Synonyms	CLEC4K
Note	A protein band of ~36 kDa is detected in mouse lung tissue lysate. Pre-incubation with the immunizing peptide completely removes langerin reactivity from NB100-56733. Confocal Microscopy: see Sun-Sang et al for details. Flow Cytometry (Intracellular): see Ness et al for details. Immunohistochemistry (frozen): see Schryver et al for details.
Reference Data
Protein Families	Druggable Genome, Transmembrane

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