TLR2 Mouse Monoclonal Antibody [Clone ID: TL2.1]

Specifications

Product Data
Clone Name	TL2.1
Applications	Block/Neutralize, Dot, ELISA, FC, ICC/IF, IP, WB
Recommend Dilution	Immunocytochemistry/ Immunofluorescence: 1:10-1:500, Immunoprecipitation: 2-5 ug, Western Blot: 1 - 2 ug/ml, Flow Cytometry: 1 ug/10^6 cells, Functional, Block/Neutralize, Flow (Cell Surface), Dot Blot, In vitro assay, ELISA
Reactivity	Human, Canine
Host	Mouse
Clonality	Monoclonal
Immunogen	This antibody was raised by immunizing mice with CHO cells transfected with human TLR2 cDNA (Flo et al, 2000). The hybridoma supernatants were selected by flow cytometry.
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	toll like receptor 2
Database Link	NP_003255 Entrez Gene 7097 Human UniProt ID O60603
Background	The Toll-like receptors (TLRs) in mammals comprise a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and an IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR2 is differentially expressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2. The expression of TLR2 in different cell types is regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes (4).
Synonyms	CD282; TIL4
Note	Immunocytochemistry/Immunofluorescence: please see Mempel et al. (2003) for details Immunohistochemistry (paraffin): please see Faure et al. (2001) for details Western Blot: please see Faure et al. (2001) for details
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Toll-like receptor signaling pathway

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