PLK1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, WB
Recommend Dilution	Immunohistochemistry, Western Blot: 1:1000-1:2000, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence
Reactivity	Human, Mouse, Rat, Bovine, Canine, Porcine, Primate
Host	Rabbit
Clonality	Polyclonal
Immunogen	A portion of amino acids 100-200 of human PLK1 was used as the immunogen.
Formulation	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Whole antisera
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	polo like kinase 1
Database Link	NP_005021 Entrez Gene 18817 MouseEntrez Gene 25515 RatEntrez Gene 5347 Human UniProt ID P53350
Background	Polo related kinase (PLK), a serine/threonine kinase is strongly correlated with the mitotic activity of cells. PLK is present in all tissues during mouse embryonic development, where as in adults expresion is limited to thymus and ovaries. It has been observed that tumors of various origin (lung, colon, stomach, smooth muscle, and esophagus as well as non-Hodgkin lymphomas) express high levels of PLK transcripts with absent of transcripts in normal surrounding tissues. Only normal colon and placenta, which are known to be mitotically active express PLK. PLK expression is restricted to proliferating cells. In mammalian cells, three PLKs (PLK1, PLK2/Snk, PLK3/Fnk) have been identified. PLK1 is the most studied isoform. The known substrates of PLK1 include several proteins involved in mitosis such as Cdc25C, APC, and cyclin B. PLK1 promotes mitosis through the phosphorylation and activation of Cdc25C, which regulates the tyrosine dephosphorylation of CDKs.
Synonyms	PLK; STPK13
Reference Data
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation

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