MBD1 Mouse Monoclonal Antibody [Clone ID: 100B272.1]

Specifications

Product Data
Clone Name	100B272.1
Applications	ChIP, ICC/IF, IHC, IP, WB
Recommend Dilution	Knockdown Validated, Chromatin Immunoprecipitation (ChIP): 1:10-1:500, Western Blot: 2-4 ug/ml, Chromatin Immunoprecipitation: 1:10-1:500, Immunoprecipitation: 1:10-1:500, Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry: 1:200, Immunohistochemistry-Paraffin: 1:200
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	This antibody was generated by immunizing mice with a synthetic peptide corresponding to amino acids 391-405 (SESEDGAGSPPPYRR) of human MBD1; GenBank no ref|NP_056671.2|
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	methyl-CpG binding domain protein 1
Database Link	NP_056669 Entrez Gene 4152 Human UniProt ID Q9UIS9
Background	DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.
Synonyms	CXXC3; PCM1; RFT
Reference Data
Protein Families	Druggable Genome, Transcription Factors

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