TRF2 (TERF2) Mouse Monoclonal Antibody [Clone ID: 4A794.15]

Specifications

Product Data
Clone Name	4A794.15
Applications	ChIP, CyTOF-ready, ELISA, FC, ICC/IF, IHC, Immunoblotting, IP, Simple Western, WB
Recommend Dilution	Chromatin Immunoprecipitation (ChIP): 1:10-1:500, Immunoblotting, Western Blot: 2-4 ug/ml, ELISA: 2 ug/ml, Immunohistochemistry-Frozen: 5 ug/ml, Immunohistochemistry-Paraffin, Simple Western: 1:50, Flow Cytometry: 0.1 ug/10^6 cells, Immunohistochemistry: 1:10 - 1:500, Immunocytochemistry/ Immunofluorescence: 1:10 - 1:500, Immunoprecipitation: 2 ug/10^6 cells, CyTOF-ready, Proximity Ligation Assay
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Baculovirus expressed whole length TRF2 protein was used for immunizing mice and splenocytes used to generate the hybridoma clone (NP_005643).
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	55.55 kDa
Gene Name	telomeric repeat binding factor 2
Database Link	NP_005643 Entrez Gene 21750 MouseEntrez Gene 361403 RatEntrez Gene 7014 Human UniProt ID Q15554
Background	TRF2 is a ubiquitously expressed protein that is implicated in the control of telomere length (1). TRF2, like TRF1 contains a Myb-related DNA binding motif. It binds to duplex TTAGGG repeats and is localized to all human telomeres in metaphase chromosomes (1). TRF2 is thought to protect chromosome ends by maintaining the correct structure at telomere termini (2). The use of mutant forms of TRF2 has implicated a role for TRF2 in the prevention of senescence in primary human cells (2). Recently, it has been shown that inhibition of TRF2 resulted in apoptosis in a subset of mammalian cell types (3)
Synonyms	TRBF2; TRF2
Note	1. TLR2 may be detected as a single band or as a doublet in western blot. Okabe (2000) described the doublet as 65 and 69 kDa using clone 4A794.15. However, observed molecular weights could vary depending on molecular weight standards used and gel conditions. 2. The TRF2 antibody is referred to as both clone 4A794.15 and 4A794 in the published literature. 3. The TRF2 antibody recognizes full-length TRF2 as well as TRF2 forms lacking both the N-terminal basic domain (B) and the telobox Myb-like C-terminal DNA-binding domain (M). TRF2 forms missing the B and M domains are often referred to as mutant TRF2. Refer to the published literature for additional information. 4. Although the exact epitope recognized by the TRF2 antibody has not been mapped, the scientific literature indicates it is in the D or L domain, but not in the B or M domain.
Reference Data
Protein Families	Transcription Factors

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