Caspase 9 (CASP9) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, IP, Simple Western, WB
Recommend Dilution	Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation: 1:50-1:200, Western Blot: 1:1000-1:2000, Immunohistochemistry-Paraffin: 1:1000-1:5000, Immunohistochemistry-Frozen, Simple Western: 1:200
Reactivity	Human, Mouse, Rat, Canine, Gerbil
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant full-length human Caspase-9 protein (pro-form) was used as an immunogen (NP_001220).
Formulation	Store at -20C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Whole antisera
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	46.3 kDa
Gene Name	caspase 9
Database Link	NP_001220 Entrez Gene 12371 MouseEntrez Gene 58918 RatEntrez Gene 842 Human UniProt ID P55211
Background	Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are synthesized as zymogens (inactive pro enzyme precursors which require a biochemical change to become active enzymes) with an N-terminal prodomain of variable length followed by a large subunit (p20) and a small subunit (p10). Caspases are activated through proteolytic cleavage at specific asparagine residues that are located within the prodomain, the p10, and p20 subunits. Activation results in the generation of mature active caspases that consist of the heterotetramer p202-p102. Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. The Caspase-9 polyclonal antisera recognizes the proform of caspase-9 (approx. 50 kDa), and the large (approx. 35 kDa) and small (approx. 15 kDa) subunits of active/cleaved Caspase-9.
Synonyms	APAF-3; APAF3; ICE-LAP6; MCH6; PPP1R56
Reference Data
Protein Families	Druggable Genome, Protease, Stem cell - Pluripotency
Protein Pathways	Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Colorectal cancer, Endometrial cancer, Huntington's disease, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Parkinson's disease, Pathways in cancer, Prostate cancer, Small cell lung cancer, VEGF signaling pathway, Viral myocarditis

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