FANCD2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Western Blot: 1:100-1:500
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to human FANCD2 (residues 217-226), surrounding the phosphorylated Serine 222. [Swiss-Prot# Q9BXW9]
Formulation	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Whole antisera
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	Fanconi anemia complementation group D2
Database Link	NP_001018125 Entrez Gene 2177 Human UniProt ID Q9BXW9
Background	FANCD2 (Fanconi anemia group D2 protein) belongs to family of FANC proteins which includes several members such as FANC-A, -B, -C, -D1/BRCA2, -D2, -E, -F, -G, -I, -J/BRIP1, -L, -M and -N/PALB2. FANCD2 interacts with several proteins such as FANCE/FANCI, USP1, MEN1, BRCA1, BRCA2, BLM, MTMR15/FAN1 and DCLRE1B/Apollo. FANC proteins (-A, -B, -C, -D1, -D2, -E, -F, -G, -L) cooperate in FANC/BRCA pathway resulting in monoubiquitination of FANCD2 which then promotes BRCA2/FANCD1 loading onto chromatin for creating nuclear foci at DNA damage sites. It is involved in DNA double-strand breaks repair, both by homologous recombination and single-strand annealing. FANCD2 is required for maintenance of chromosomal stability and promotes accurate/efficient pairing of homologs during meiosis. FANCD2 may participate in S phase/G2 phase checkpoint activation upon DNA damage amd plays a role in preventing breakage and loss of missegregating chromatin at the end of cell division, particularly after replication stress. Defects in FANCD2 are a cause of Fanconi anemia complementation group D type 2 characterized by anemia, leukopenia and thrombopenia.
Synonyms	FA-D2; FA4; FACD; FAD; FAD2; FANCD
Note	This FANCD2 [p Ser222] antibody is useful for Western Blot. 1:400 dilution recommended for PD20 FANCD2 overexpressing cells, 1:100 for HeLa cells, 1:200 for other cell types.
Reference Data
Protein Families	Druggable Genome

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