Calnexin (CANX) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, ICC/IF, IHC, IP, Simple Western, WB
Recommend Dilution	Flow Cytometry, Western Blot: 1:1000, Immunohistochemistry-Paraffin: 1:100, Simple Western: 1:50, Immunohistochemistry: 1:100, Immunocytochemistry/ Immunofluorescence: 1:100, Immunoprecipitation: 1:100
Reactivity	Human, Mouse, Rat, Hamster
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to a C-terminal portion of the rat Calnexin protein (between residues 550-591) [UniProt P35565]
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	97 kDa
Gene Name	calnexin
Database Link	NP_001737 Entrez Gene 12330 MouseEntrez Gene 29144 RatEntrez Gene 821 Human UniProt ID P27824
Background	Calnexin is a calcium-binding protein which interacts with newly synthesized glycoproteins in ER for facilitating protein assembly and/or the retention within ER of unassembled protein subunits as well as incorrectly folded proteins thereby playing a key role in ER's quality control apparatus. The specificity of calnexin for glycoproteins is defined by a lectin site, which binds an early oligosaccharide intermediate on the folding glycoprotein. When associated with partial T-cell antigen receptor complexes which escape immature thymocytes's ER, calnexin regulates thymocyte maturation process and is also implicated in receptor-mediated endocytosis at synapses. MAPK3/ERK1 phosphorylates calnexin at Ser-565 leading to increased calnexin-ribosomal association and DHHC6 mediated calnexin palmitoylation leads to its preferential localization to perinuclear rough ER. Palmitoylation also facilitate calnexin association with RTC/ribosome-translocon complex that is essential to efficient folding of glycosylated proteins.
Synonyms	CNX; IP90; P90
Note	This Calnexin antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western Blot, and Immunohistochemistry-paraffin embedded sections. In ICC/IF, endoplasmic reticulum staining was observed in HeLa cells. In Western Blot, a band is seen ~90 kDa representing Calnexin. In IHC-P, staining was observed in the endoplasmic reticulum of mouse bladder tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Antigen processing and presentation

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