Chk1 (CHEK1) Mouse Monoclonal Antibody [Clone ID: 2G1D5]

Specifications

Product Data
Clone Name	2G1D5
Applications	ICC/IF, IHC, Simple Western, WB
Recommend Dilution	Simple Western: 1:100, Western Blot: 1:200 - 1:1000, Immunocytochemistry/ Immunofluorescence: 1:10 - 1:500, Immunohistochemistry: 1:250, Immunohistochemistry-Paraffin: 1:250
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of Chk1 expressed in E. coli. [UniProt# O14757]
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	56 kDa
Gene Name	checkpoint kinase 1
Database Link	NP_001265 Entrez Gene 1111 Human UniProt ID O14757
Background	Chk1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by Chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Chk1 can also phosphorylate p53 at Ser20 in vitro.
Synonyms	CHK1
Note	This Chk1 (2G1D5) antibody is useful for Western blot and ELISA.
Reference Data
Protein Families	Druggable Genome, Protein Kinase, Stem cell - Pluripotency
Protein Pathways	Cell cycle, p53 signaling pathway

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.