RAD50 Rabbit Polyclonal Antibody

CAT#: TA336262

Rabbit Polyclonal Rad50 Antibody



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CNY 5,381.00


货期*
5周

规格
    • 100 ul

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Specifications

Product Data
Applications ICC/IF, IF, IP, Simple Western, WB
Recommend Dilution ICC/IF (Negative), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation: 1:10-1:500, Western Blot: 1:2000, Simple Western: 1:2000
Reactivity Human, Mouse, Hamster
Host Rabbit
Clonality Polyclonal
Immunogen C-terminal mouse RAD50 (604 amino acids). [UniProt# P70388]
Formulation Store at 4C. Do not freeze.
Concentration lot specific
Purification Whole antisera
Conjugation Unconjugated
Storage Condition Store at -20°C as received.
Predicted Protein Size 153 kDa
Gene Name RAD50 double strand break repair protein
Background RAD50 (DNA repair protein RAD50) is a component of MRN complex (Mre11-Rad50-Nbs1) that plays an important role in detection and signaling of DNA double strand breaks (DSBs) through acting as DSB sensor, co-activator of DSB-induced cell cycle checkpoint signaling, and as repair-effector in two competing DSB repair pathways: homologous recombination (HR) and non-homologous end-joining (NHEJ). MRN complex also associates with telomeres at the ends of linear chromosomes, where it contributes to their maintenance. RAD50 is required to bind DNA ends for holding them in close proximity where it facilitates searches for short or long regions of sequence homology in the recombining DNA templates, and also stimulate the activity of DNA ligases and/or restrict MRE11A's nuclease activity to prevent nucleolytic degradation. DSBs can be caused by ionizing radiation, certain chemotherapy drugs, metabolic ROS, as errors during replication, by programmed enzymatic activities during meiosis/V(D)J recombination etc., and if left unrepaired, DSBs can generate chromosomal translocations, aneuploidy and carcinogenesis. Defects in RAD50 have been linked to Nijmegen breakage syndrome-like disorder (NBSLD).
Synonyms hRad50; NBSLD; RAD502
Note This RAD50 antibody is useful for Immunoprecipitation and Western blot, where a band can be seen at 153 kDa. IP has been done with 3-4 X 10(6) cells. ICC/IF does not appear to work. Immunocytochemistry/Immunofluorescence was reported in scientific literature.
Reference Data
Protein Families Druggable Genome
Protein Pathways Homologous recombination, Non-homologous end-joining
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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