Collagen IV (COL4A3) Goat Polyclonal Antibody

Specifications

Product Data
Applications	IHC
Recommend Dilution	IHC: 4-6ug/ml
Reactivity	Human, Dog, Cow
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence C-RGPTEYYDTYQEK, from the internal region of the protein sequence according to NP_000082.2
Formulation	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration	lot specific
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	collagen type IV alpha 3 chain
Database Link	NP_000082 Entrez Gene 403842 DogEntrez Gene 1285 Human UniProt ID Q01955
Background	Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jun 2010]
Synonyms	alpha-3 polypeptide; alpha 3 (Goodpasture antigen); alpha 3 type IV collagen; alpha3 type IV collagen; collagen; collagen IV; Goodpasture antigen; OTTHUMP00000195044; TUMSTATIN; tumstatin; type IV
Note	IHC: In paraffin embedded Human Kidney shows staining at the luminar side of the epithelial cell membranes.
Reference Data
Protein Families	Druggable Genome

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