Ryanodine Receptor (RYR1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	WB: 1:200-1:2000; IHC: 1:100-1:3000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)RAENEKDATTEKNKKR, corresponding to amino acid residues 1371-1386 of human Ryanodine Receptor 1.? Intracellular, N-terminus.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	ryanodine receptor 1
Database Link	NP_000531 Entrez Gene 20190 MouseEntrez Gene 114207 RatEntrez Gene 6261 Human UniProt ID P21817
Background	It is well established that cytosolic calcium (Ca2+) acts as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The primary intracellular Ca2+ storage/release organelle in most cells is the endoplasmic reticulum (ER) or the sarcoplasmic reticulum (SR) in striated muscle cells. The ER and SR contain two Ca2+ release channels families, the Inositol trisphosphate receptors (IP3Rs) and the Ryanodine receptors (RyRs). The Ryanodine receptor family consists of three different isoforms: The skeletal muscle isoform, Ryanodine Receptor type 1 (RyR1); the cardiac muscle isoform, Ryanodine Receptor type 2 (RyR2) and the brain isoform, Ryanodine Receptor type 3 (RyR3). The Ryanodine receptors are homotetrameric proteins generating a Ca2+ conducting channel . They play a key role in the mechanism of excitation-contraction coupling in striated muscle. Binding of Ryanodine to the Ryanodine Receptor causes to two major changes in the channel: a reduction in single-channel conductance and a marked increase in open state probability. The RyR1 is expressed predominantly in skeletal muscles and areas of the brain; the human RyR1 has at least three known alternative spliced variants. Several diseases are attributed to mutations in RyR1 gene: Central Core Disease (CCD), multi-minicore disease (MmD) and Malignant Hyperthermia (MH).
Synonyms	CCO; MHS; MHS1; PPP1R137; RYDR; RYR; RYR-1; SKRR
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Calcium signaling pathway, Long-term depression

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