Grm8 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	WB: 1:200-1:2000; IHC: 1:100-1:3000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)ENFG(S)KLGSHGKR, corresponding to amino acid residues 365- 377 of rat mGluR8. Extracellular, N-terminus.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	glutamate metabotropic receptor 8
Database Link	NP_071538 Entrez Gene 2918 HumanEntrez Gene 14823 MouseEntrez Gene 60590 Rat UniProt ID P70579
Background	Metabotropic glutamate receptors (mGluRs), heptahelical transmembrane receptors coupled to G-proteins, contribute to the regulation of neuronal excitability and synaptic transmission. Genes coding for eight mGluRs (denoted mGluR1-8) with different splice variants have been identified and classified into three groups based on sequence homology, pharmacology, and signaling pathways. Group I mGluRs (mGluR1 and mGluR5) are primarily located postsynaptically. Group II (mGluR2 and mGluR3) are present both postsynaptically and presynaptically outside the release site. Group III mGluRs (mGluR4, mGluR6, mGluR7, and mGluR8) couple to Gai/o to inhibit adenylyl cyclase found in the presynaptic active zone. The mGluR8 receptor is detected in the olfactory bulb, thalamus, pontine gray, cerebral cortex, hippocampus, cerebellum, and retina. Electrophysiological studies suggest that mGluR8 functions as a presynaptic autoreceptor to regulate glutamate release from the lateral perforant path terminals in the mouse dentate gyrus. It is suggested that mGluR8, like other group III mGluRs, controls glutamate release by inhibiting voltage-gated Ca2+ channels. Targeted deletion of the mGluR8 gene in mice produces subtle behavioral alterations such as reduced habituation to novelty, hyperactivity, context-dependent but not cue-dependent disruption of the freezing response in the fear conditioning test, and increased anxiety.
Synonyms	FLJ41058; GLUR8; GPRC1H; MGC126724; mGlu8; MGLUR8; OTTHUMP00000024506; OTTHUMP00000069010; OTTHUMP00000199504
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.