Gabra4 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	WB: 1:200-1:2000; IHC: 1:100-1:3000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide NSKDEKLSPENFTR(C), corresponding to amino acid residues 37-50 of rat GABA(A) a4 with replacement of C44 with serine. Extracellular, N-terminus.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	gamma-aminobutyric acid type A receptor alpha4 subunit
Database Link	NP_542154 Entrez Gene 2557 HumanEntrez Gene 14397 MouseEntrez Gene 140675 Rat UniProt ID P28471
Background	GABA (?-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake, and metabolism all occur in the nervous system.The GABA transmitter interacts with two major types of receptors: ionotropic GABAA receptors (GABAAR) and metabotropic receptors (GABABR). GABAARs belong to the ligand-gated ion channel superfamily. GABA inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells. Binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance that results in inhibition of neural activity.GABAARs are heteropentamers, in which all five subunits contribute to the pore formation. To date, eight subunit isoforms have been cloned: a, Ã?, ?, d, e, p, ?, and ?. Six a subunit isoforms have been found to exist in mammals (a1-a6). In most cases, native GABAA receptors consist of 2a, 2Ã?, and 1? subunits. The a subunit is the most common and is expressed ubiquitously. It determines the affinity of GABAARs for allosteric ligands.Each subtype has a unique regional expression in the brain, and individual neurons often express multiple subtypes. For example, the a4 subunit is detected in the hippocampus, cortex, olfactory bulb and in the basal forebrain.
Synonyms	GABRA4
Note	This antibody was tested in live cell imaging. Please see IF/ICC data for detail.
Reference Data

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