Cntfr Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:200-1:2000
Reactivity	Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide CHIRYMHLFSTIKYK, corresponding to amino acid residues 164-178 of rat CNTFRa . Extracellular.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	ciliary neurotrophic factor receptor
Database Link	NP_001003929 Entrez Gene 12804 MouseEntrez Gene 313173 Rat UniProt ID Q08406
Background	CNTF is a polypeptide trophic factor, member of the alpha-helical cytokine superfamily. It was initially purified from the chick eye on the basis of its ability to promote survival of E8 chick ciliary ganglion neurons in culture. CNTF is synthesized by glia both in the CNS and PNS3 and it has been demonstrated that CNTF is ubiquitously distributed in neurons and glia throughout the rodent brain. CNTF effects are mediated by a tripartite receptor complex consisting of two signal-transducing subunits (leukemia inhibitory factor receptor, gp130) and a CNTF-specific ligand-binding-subunit (CNTFRa).CNTFRa is anchored to the membrane by a glycosyl-phosphatidylinositol (GPI) linkage and lacks the intracellular signaling moiety. It can be cleaved from the GPI anchor to yield a soluble form of the receptor (sCNTFRa).CNTF binds to CNTFRa, the latter associates with gp130 which recruits LIFR. This leads to the activation of a signaling cascade involving the JAK/STAT pathway.CNTFRa knockout mice die within 24 hours after birth as opposed to CNTF knock animals which are viable. This strongly suggest that CNTFRa has other ligands besides CNTF. Indeed, CLC/CLF complex (cardiotrophin like cytokine/cytokine-like factor-1) binds CNTFRa and activates a similar signaling pathway to that of CNTF.
Synonyms	MGC1774
Reference Data

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