Clic5 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:200-1:2000
Reactivity	Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)DTNTHGDEKGSQRK, corresponding to amino acid residues 161- 174 of rat CLIC5. Intracellular, C-terminus.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	chloride intracellular channel 5
Database Link	NP_446055 Entrez Gene 224796 MouseEntrez Gene 94272 Rat UniProt ID Q9EPT8
Background	Chloride (Cl) channels are membrane, anion-selective protein pores, which diffusively transport negative ions across their electrochemical gradient. Cl channels are subdivided into six unique families, and their regulation mechanisms range from voltage-dependency, through G-protein activation, to mechanosensitivity; they may reside in either the plasma membrane or the membrane of intracellular compartments or both. They are detected in the kidney, placenta, intestines, brain and the inner-ear. The vertebrate Cl intracellular channel (CLIC) family is composed of 6 highly conserved members (CLIC1-6); each exists as a ~250 residue protein which can assume both a soluble fold and a membrane-integral form. The latter consists of a single trans-membrane domain and is subsequently oligomerized to construct a functioning channel. Interestingly, channels composed of CLIC4 and CLIC5 have been confirmed to be equally permeable to K+ and Cl. CLIC5, to which there are two isoforms - CLIC5A and CLIC5B - encoded by the same gene, has first been isolated from human placenta, and was shown, together with CLIC4 (with which it shares 76% homology), to be enriched in the apical microvilli-containing part of the trophoblast epithelium, where, unlike CLIC4, it interacts with the actin cytoskeleton. CLIC5 shares about 40% homology with CLIC1-4 and is distributed to a high degree in myocytes, cardiomyocytes and stereocillia of the inner-ear. Mice mutated in the Clic5 gene lack coordination and gradually become deaf, show resistance to obesity, experience gastric ulcers and the resultant hemorrhage, and have higher incidence of entering torpor.
Synonyms	CLIC5B; dJ447E21.4; FLJ90663; MST130; MSTP130
Reference Data

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