Rabbit Monoclonal Antibody

Specifications

Product Data
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Immunogen	GST fusion protein with residues 1-46 of rabbit Cav1.2a, with serine 44 replaced with alanine. Intracellular, N-terminus.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST, and then anti-Cav1.2a antibody was affinity purified on immobilized CardN1-46(S44A).
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Background	All L-type calcium channels are encoded by one of the CaV1 channel genes. These channels play a major role as a Ca2+ entry pathway in skeletal cardiac and smooth muscles as well as in neurons, endocrine cells and possibly in non-excitable cells such as hematopoetic and epithelial cells. Several peptidyl toxins are described that are specific L-type channels blockers, but so far no selective blocker for one of the CaV1 isoforms have been described. These include the Mamba toxins Calcicludine, Calciseptine and FS-2. All CaV1 channels are influenced by dIHCydropyridines (DHP) and are also referred to as DHP receptor. While the CaV1.1 and CaV1.4 isoforms are expressed in restricted tissues (skeletal muscle and retina, respectively), CaV1.3 channels are found in heart, brain and pancreas and CaV1.2 channels are ubiquitously expressed. There are two splice variants to the Cav1.2 channel designated Cav1.2a and Cav1.2b. The expression of the Cav1.2b variant is restricted to smooth muscle while Cav1.2a specifically expressed in cardiac muscle.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.