Adora2a Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	WB: 1:200-1:2000; IHC: 1:100-1:3000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)RQLKQMESQPLPGER, corresponding to amino acid residues 201-215 of mouse A2A Adenosine receptor. 3rd intracellular loop.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	adenosine A2a receptor
Database Link	NP_033760 Entrez Gene 135 HumanEntrez Gene 25369 RatEntrez Gene 11540 Mouse UniProt ID Q60613
Background	Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions of many tissues including brain and heart. Adenosine exerts its action via four specific adenosine receptors (also named P1 purinergic receptors): A1-Adenosine Receptor (A1AR), A2A-Adenosine Receptor (A2AAR), A2B-Adenosine Receptor (A2BAR), and A3-Adenosine Receptor (A3AR). All are integral membrane proteins and are members of the G protein-coupled receptor superfamily, which share a common structure of seven putative transmembrane domains, an extracellular NH2 terminus, cytoplasmic COOH terminus, and a third intracellular loop important for binding G proteins. The adenosine receptors can be distinguished on the basis of their differential selectivity for adenosine analogs. Adenosine receptors control neurotransmitter release through the facilitatory A2AAR and the inhibitory A1AR. A2AAR and A1AR are the major adenosine receptor subtypes expressed in the central nervous system (CNS). A2AAR is mainly expressed in the striatum on GABAergic striatopallidal neurons, while A1AR is widely distributed throughout the CNS. A2AAR was suggested to play a critical role in attenuation of systemic inflammatory responses and prevention of extensive tissue damage. It was suggested that extracellular adenosine that accumulates in inflamed and damaged tissue may activate the A2AAR expressed in immune cells leading to termination/inhibition of the immune response. It was further suggested that this same mechanism may protect tumors from antitumor T cells through an immunosuppressive signal generated by the activation of A2AAR on T cells by extracellular adenosine produced from hypoxic cancerous tissues.
Synonyms	ADORA2; hA2aR; RDC8
Reference Data

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