GABA A Receptor alpha 3 (GABRA3) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	WB: 1:200-1:2000; IHC: 1:100-1:3000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide QGESRRQEPGDFVKQ(C), corresponding to amino acid residues 29-43 of human GABA (A) a3 Receptor. Extracellular, N-terminus.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	gamma-aminobutyric acid type A receptor alpha3 subunit
Database Link	NP_000799 Entrez Gene 14396 MouseEntrez Gene 24947 RatEntrez Gene 2556 Human UniProt ID P34903
Background	The neurotransmitter GABA (?-aminobutiric acid) inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells. Binding of GABA to its GABAA receptor results in conformational changes that open a Cl channel, producing an increase in membrane conductance that results in inhibition of neural activity. There are two major types of GABA receptors: the ionotropic GABAA receptors (GABAAR) and the metabotropic GABAB receptors (GABABR). GABAARs belong to the ligand-gated ion channel superfamily. GABAARs are heteropentamers, in which all five subunits contribute to formation of the pore. Eight subunit isoforms have been cloned: a, Ã?, ?, d, e, p, ?, and ?.Six a subunits isoforms (a1-a6) have been shown to exist in mammals. In most cases, the native GABAA receptors consist of 2a, 2Ã?, and 1? subunits. The a3-subunit is highly expressed during development (along with a2 and a5) and then declines in adulthood, where the a1-subunit becomes predominant. The failure to complete this switch could be a major predispositional factor in the development of temporal lobe epilepsy.
Synonyms	MGC33793
Note	This antibody was tested in live cell imaging. Please see IF/ICC data for detail.
Reference Data
Protein Families	Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
Protein Pathways	Neuroactive ligand-receptor interaction

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