MC1 Receptor (MC1R) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	WB: 1:200-1:2000; IHC: 1:100-1:3000
Reactivity	Human, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)HAQGIARLHKRQRPVH, corresponding to amino acid residues 217-232 of human MC1R. 3rd intracellular loop.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: Phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.025% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	melanocortin 1 receptor
Database Link	NP_002377 Entrez Gene 102552838 RatEntrez Gene 4157 Human UniProt ID Q01726
Background	Melanocortin Receptor 1 (MC1R) belongs to a five-member receptor family known as the melanocortin receptors. The melanocortin receptors are members of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. The receptors ligands, the melanocortins, are a group of structurally derived peptides consisting of a-, Ã?- and ?-melanocyte stimulating hormone (a, Ã?, ?-MSH) and the adrenocorticotropic hormone (ACTH) all of which are derived from the post-translational processing of a common precursor peptide, proopiomelanocortin (POMC). One of the most salient features of the melanocortin signaling system is the presence of two endogenous antagonists, that is proteins that bind specifically to the receptor but instead of activating it have an inhibitory effect. The antagonist proteins are termed agouti (or agouti signaling protein, ASP) and agouti-related protein (AGRP). All five melanocortin receptors bind their agonists (the melanocortins) and their endogenous antagonists (agouti and AGRP) with different affinities. MC1R was the first member of the melanocortin receptor family to be cloned. The receptor transduces signals via Gs resulting in the activation of adenylate cyclase and production of cAMP. MC1R binds its ligands with the following potency: a-MSH = ACTH > Ã?-MSH > ?-MSH. MC1R also binds the endogenous antagonist agouti with high affinity. MC1R can be described as the â??classicalâ? melanocyte a-MSH receptor. The receptor is expressed in the skin where it has a key role in determining skin and hair pigmentation. In fur-bearing mammals, the local ratio between a-MSH and agouti will determine coat pigmentation as a-MSH stimulates and agouti inhibits melanin production. In humans, especially in Caucasians, the MC1R gene is highly polymorphic and several allelic variants have been correlated with red-hair, poor tanning ability and increased risk of melanoma. In addition, MC1R expression in melanoma has been shown to be upregulated up to 20-fold when compared to normal melanocytes. The melanocortins and particularly a-MSH have significant anti-inflammatory properties. Since a-MSH binds to MC1R with the highest potency, it was proposed that the latter mediated the anti-inflammatory effects of a-MSH. Indeed, MC1R expression has been demonstrated in several cells of the immune system including macrophages and neutrophils.
Synonyms	CMM5; MSH-R; SHEP2
Reference Data
Protein Families	Druggable Genome, GPCR, Transmembrane
Protein Pathways	Melanogenesis, Neuroactive ligand-receptor interaction

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