TPX2 Mouse Monoclonal Antibody [Clone ID: 18D5]

Specifications

Product Data
Clone Name	18D5
Applications	WB
Recommend Dilution	WB, IHC, IF
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Amino Acid: 1-220 of human TPX2
Formulation	This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Concentration	lot specific
Purification	The antibody was purified by affinity chromatography.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	100 kD
Gene Name	TPX2, microtubule nucleation factor
Database Link	NP_036244 Entrez Gene 22974 Human UniProt ID Q9ULW0
Background	The TPX2 protein, also known as targeting protein for Xklp1, restricted expression proliferation associated protein p100, and differentially expressed in cancer and non-cancerous lung cells protein 2 (DIL2), is a 100 kD nuclear protein that contains two coiled-coil domains. The TPX2 protein is strictly associated with spindle pole and mitotic spindle during mitosis. In the G2/S position of the cell cycle, TPX2 is diffusely distributed throughout nucleus. TPX2 has been shown to be highly expressed in lung carcinomas cell lines, but not in normal lung tissues. TPX2 is thought to be required for the Ran-GTP dependent assembly of microtubules around chromosomes required to generate stable bipolar spindle with overlapping anti-parallel microtubule arrays and may also be involved in targeting Aurora-A kinase to the mitotic spindle. TPX2 has been shown to interact with a large number of proteins including serine/threonine protein kinase 6, ribosomal protein 6, Bop-1, α-tubulin, and nucleolin among others. TPX2 can be modified by phosphorylation on serine 738. The 18D5 monoclonal antibody recognizes human TPX2 and has been shown to be useful for Western blotting.
Synonyms	C20orf1; C20orf2; DIL-2; DIL2; FLS353; GD:C20orf1; HCA519; HCTP4; p100; REPP86
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency

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