PARP1 Mouse Monoclonal Antibody [Clone ID: 5A5]

Specifications

Product Data
Clone Name	5A5
Applications	IF, WB
Recommend Dilution	IF, WB
Reactivity	Mouse, Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant (partial), N-terminal 2/3 sequence of PARP
Formulation	This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Concentration	lot specific
Purification	The antibody was purified by affinity chromatography.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	113 kD
Gene Name	poly(ADP-ribose) polymerase 1
Database Link	NP_001609 Entrez Gene 11545 MouseEntrez Gene 142 Human UniProt ID P09874
Background	PARP (Poly (ADP-ribose) polymerase) is a 113 kD nuclear protein that can exist as a homo- or hetero-dimer. This protein acts as a molecular "nick sensor" and functions in base excision repair, poly(ADP-ribosyl)ation of acceptor proteins involved in chromatin architecture and DNA metabolism and participates in protein modification to enhance or repress transcription. PARP is ribosylated by PARP2 and is a target for caspase cleavage during apoptosis. PARP interacts with proteins in the base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. In addition PARP forms heterodimers with PARP2, and interacts with PARP3. The 5A5 monoclonal antibody recognizes the N-terminal region of human and mouse PARP and has been shown to be useful for Western blotting and immunofluorescence staining.
Synonyms	ADPRT; ADPRT 1; ADPRT1; ARTD1; pADPRT-1; PARP; PARP-1; PPOL
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency, Transcription Factors
Protein Pathways	Base excision repair

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