BRG1 (SMARCA4) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, IP, WB
Recommend Dilution	WB 1:500 - 1:2000;IF 1:50 - 1:200;IP 1:20 - 1:50
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide of human SMARCA4
Formulation	Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Concentration	lot specific
Purification	Affinity purification
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	185 kDa
Gene Name	SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Database Link	NP_001122321 Entrez Gene 20586 MouseEntrez Gene 171379 RatEntrez Gene 6597 Human UniProt ID P51532
Background	The modulation of chromatin structure is an essential component in the regulation of transcriptional activation and repression. Modifications can be made by at least two evolutionarily conserved strategies, through the disruption of histone-DNA contacts by ATP-dependent chromatin remodelers, or by histone tail modifications including methylation and acetylation. One of the four classes of ATP-dependent histone remodelers is the SWI/SNF complex, the central catalytic subunit of which is Brg1 or the highly related protein hBRM . This SWI/SNF complex contains varying subunits but its association with either Brg1 or hBRM remains constant . SWI/SNF complexes have been shown to regulate gene activation, cell growth, the cell cycle and differentiation . Brg1/hBRM have been shown to regulate transcription through enhancing transcriptional activation of glucocorticoid receptors . Although usually associated with transcriptional activation, Brg1/hBRM have also been found in complexes associated with transcriptional repression including with HDACs, Rb and Tif1β . Brg1/hBRM plays a vital role in the regulation of gene transcription during early mammalian embryogenesis. In addition, Brg1/hBRM also play a role as a tumor suppressors and Brg1 is mutated in several tumor cell lines.
Synonyms	BAF190; BAF190A; BRG1; CSS4; hSNF2b; MRD16; RTPS2; SNF2; SNF2L4; SNF2LB; SWI2
Reference Data
Protein Families	Druggable Genome, Transcription Factors

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