ERK5 (MAPK7) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, WB
Recommend Dilution	WB 1:500 - 1:2000;IF 1:50- 1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant protein of human MAPK7
Formulation	Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Concentration	lot specific
Purification	Affinity purification
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	mitogen-activated protein kinase 7
Database Link	NP_002740 Entrez Gene 23939 MouseEntrez Gene 114509 RatEntrez Gene 5598 Human UniProt ID Q13164
Background	ERK5 (Mitogen-activated protein kinase 7, Big mitogen-activated protein kinase 1) is a member of the MAPK superfamily implicated in the regulation numerous cellular processes including proliferation, differentiation, and survival. Like other MAPK family members, ERK5 contains a canonical activation loop TEY motif (Thr218/Tyr220) which is specifically phosphorylated by MAP2K5 (MEK5) in a growth factor-dependent, Ras-independent mechanism. For example, EGF stimulation promotes ERK5 phosphorylation which induces its traslocation to the nucleus where it phosphorylates MEF2C and other transcriptional targets. ERK5 is also activated in response to granulocyte colony-stimulating factor (G-CSF) in hematopoetic progenitor cells where it promotes survival and proliferation. In neuronal cells, ERK5 is required for NGF-induced neurite outgrowth, neuronal homeostatis, and survival. ERK5 is thought to play a role in blood vessel integrity via maintainence of endothelial cell migration and barrier function. Although broadly expressed, research studies have shown that mice lacking erk5 display numerous cardiac defects, suggesting ERK5 plays a critical role in vascular development and homeostasis.
Synonyms	BMK1; ERK4; ERK5; PRKM7
Reference Data
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Gap junction, GnRH signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway

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