14-3-3 epsilon (YWHAE) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, WB
Recommend Dilution	WB 1:500 - 1:2000;IF 1:20 - 1:100
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant protein of human YWHAE
Formulation	PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Concentration	lot specific
Purification	Affinity purification
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	29 kDa
Gene Name	tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon
Database Link	NP_006752 Entrez Gene 22627 MouseEntrez Gene 29753 RatEntrez Gene 7531 Human UniProt ID P62258
Background	The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, ?, e, s, ?, t, and ? that have been identified in mammals. The initially described a and d isoforms are confirmed to be phosphorylated forms of β and ?, respectively. Through their amino-terminal a helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules. The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation-independent interactions, has been observed. 14-3-3 binding masks specific sequences of the target protein and therefore modulates target protein localization, phosphorylation state, stability, and molecular interactions. 14-3-3 proteins may also induce target protein conformational changes that modify target protein function. Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities. Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes.
Synonyms	14-3-3E; HEL2; KCIP-1; MDCR; MDS
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis

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