Amyloid Precursor Protein (APP) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	WB: 1:500-1:2000
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	N term -peptide of human APP
Formulation	Store at -20C or -80C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Concentration	lot specific
Purification	Affinity purification
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	amyloid beta precursor protein
Database Link	NP_000475 Entrez Gene 351 Human UniProt ID P05067
Background	Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDatransmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain (Aβ), which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques in Alzheimer's disease (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (2-5). Phosphorylation at Thr668 (at a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell cycle-dependent and peaks during G2/M-phase (4). APP phosphorylated at Thr668 exists in adult rat brain and correlates with cultured neuronal differentiation (5,6).1. Selkoe, D.J. (1996) J Biol Chem 271, 18295-8.2. Caporaso, G.L. et al. (1992) Proc Natl Acad Sci USA 89, 3055-9.3. Hung, A.Y. and Selkoe, D.J. (1994) EMBO J 13, 534-42.4. Suzuki, T. et al. (1994) EMBO J 13, 1114-22.5. Ando, K. et al. (1999) J Neurosci 19, 4421-7.6. Iijima, K. et al. (2000) J Neurochem 75, 1085-91.
Synonyms	AAA; ABETA; ABPP; AD1; APPI; CTFgamma; CVAP; PN-II; PN2
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Alzheimer's disease

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