Osteopontin (SPP1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, WB
Recommend Dilution	SPP1 antibody can be used for detection of SPP1 by Western blot at 1 - 2 µg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	SPP1 antibody was raised against an 18 amino acid peptide near the amino terminus of human SPP1.
Specificity	SPP1 antibody is human, mouse and rat reactive. Multiple isoforms of SPP1 are known to exist.
Formulation	SPP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/ml
Purification	SPP1 Antibody is Protein A purified.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	Predicted: 36 kDa; Observed: 37 kDa
Gene Name	secreted phosphoprotein 1
Database Link	NP_001238759 Entrez Gene 6696 Human UniProt ID P10451
Background	SPP1 Antibody: The secreted protein 1 (SPP1), also known as osteopontin, is a major noncollagenous protein of bone, but is also found in the extracellular matrix of other mineralized tissues and in bodily fluids. In bone, SPP1 is produced by osteoblasts, osteocytes, macrophages, and osteoclasts (1,2). SPP1 binds to cells through integrin and non-integrin receptors, as well as the adhesion receptor CD44 in an RGD-independent manner, enabling SPP1 to induce a number of functional effects including macrophage chemotaxis, cytoprotection, and regulation of T helper type 1 cells (2). SPP1 can regulate biomineralization by inhibiting the formation of hydroxyapatite (3) and the growth of calcium oxalate crystals (4).
Synonyms	BNSP; BSPI; ETA-1; OPN
Reference Data

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