HSF1 Rat Monoclonal Antibody [Clone ID: 10H8]

Specifications

Product Data
Clone Name	10H8
Applications	IF
Recommend Dilution	1ug/ml was sufficient for detection of HSF1 by ECL immunoblot in 20ug of HeLa lysate.
Reactivity	Human, Mouse, Rat, Bovine, Guinea Pig, Hamster, Rabbit, Monkey
Host	Rat
Clonality	Monoclonal
Immunogen	Purified recombinant HSF1 of mouse origin, epitope mapping to amino acids 378-395
Formulation	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration	lot specific
Purification	Protein G Purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	heat shock transcription factor 1
Database Link	NP_005517 Entrez Gene 15499 MouseEntrez Gene 79245 RatEntrez Gene 714078 MonkeyEntrez Gene 3297 Human UniProt ID Q00613
Background	HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function . The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) . Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the Nterminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization . There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally active than the smaller beta form . Various experiments have suggested that HFS2 may have roles in differentiation and development .
Synonyms	HSTF1
Note	Detects an ~85kDa protein in unstressed cell lysates, and an ~95kDa protein in heat shocked cell lysates, corresponding to the molecular mass of inactive and active forms of HSF1 on SDS PAGE immunoblots.
Reference Data
Protein Families	Transcription Factors

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