Nucleostemin (GNL3) Mouse Monoclonal Antibody [Clone ID: 3C12-H11]

Specifications

Product Data
Clone Name	3C12-H11
Applications	IHC, WB
Recommend Dilution	WB: 1:2000
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	His-tagged human recombinant hsp22
Formulation	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration	lot specific
Purification	Protein G Purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	G protein nucleolar 3
Database Link	NP_996562 Entrez Gene 30877 MouseEntrez Gene 290556 RatEntrez Gene 26354 Human UniProt ID Q9BVP2
Background	Hsp22 (HSPB8) is a 196-amino acid protein that is a member of the small heat shock protein super-family and the human protein is most closely related to Hsp27. Similar to most other sHSPs, Hsp22 is predominately transcribed in skeletal muscle and heart, as well as the placenta . Hsp22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. In a two hybrid screen, HspB8 interacted preferentially with a triple aspartate form of Hsp27 which mimics Hsp27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type Hsp27 . HSPB8 has two binding domains (N and C Terminal) that are specific for different binding partners, and has the ability to bind itself and other sHSPs . The chaperone-like activity is of great importance to the function of Hsp22 in various processes including proliferation, apoptosis and macroautophagy . Mutations in the HSPB8 gene are associated with the inherited peripheral neurpathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L) .
Synonyms	C77032; E2IG3; NNP47; NS
Note	Detects endogenous and exogenous hsp22 in monomeric, dimeric and tetrameric forms in WB. Does not cross react with alpha crystallin.
Reference Data
Protein Families	ES Cell Differentiation/IPS, Stem cell - Pluripotency

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