Heme Oxygenase 1 (HMOX1) Mouse Monoclonal Antibody [Clone ID: 1F12-A6]

Specifications

Product Data
Clone Name	1F12-A6
Applications	WB
Recommend Dilution	WB: 1:500-1000
Reactivity	Human, Mouse, Bovine
Host	Mouse
Clonality	Monoclonal
Immunogen	Human HO-1 synthetic peptide, amino acids 1-30
Formulation	PBS pH7.4, 50% glycerol
Concentration	lot specific
Purification	Protein G Purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	heme oxygenase 1
Database Link	NP_002124 Entrez Gene 15368 MouseEntrez Gene 3162 Human UniProt ID P09601
Background	Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin . These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs .There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals . HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation . It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency , susceptibility to atherosclerotic lesion formation , endothelial cell injury, and growth retardation . Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress .
Synonyms	bK286B10; HMOX1D; HO-1; HSP32
Note	Detects a 32kDa protein, corresponding to the molecular mass of HO-1 on SDS Page immunoblots. Does not cross-react with HO-2
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Porphyrin and chlorophyll metabolism

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