HMGB4 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 200-1000 WB positive control: 231 cells and human fetal liver tissue
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Full length fusion protein
Formulation	PBS pH7.3, 0.05% NaN3, 50% glycerol
Concentration	lot specific
Purification	Antigen affinity purification
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	22 kDa
Gene Name	high mobility group box 4
Database Link	NP_660206 Entrez Gene 127540 Human UniProt ID Q8WW32
Background	High mobility group protein B4?is a?transcription factor?that in humans is encoded by the?HMGB4?gene.HMGB4 is strongly and preferentially expressed in the adult mouse testis and weakly in the brain; but not in many other tissues. HMGB4 associates with chromatin; and in transfection assays; in contrast to HMGB1; it acts as a potent transcriptional repressor. During spermatogenesis; HMGB4 is present in the euchromatin of late pachytene spermatocytes and haploid round spermatids; whereas stronger expression is observed during the elongation phase; where it localizes to the basal pole of the nucleus in a manner mutually exclusive with H1FNT (H1T2) localized at the apical pole. HMGB4 basal localization is lost in H1FNT-mutant spermatids; showing that H1FNT provides a positional cue for organizing chromatin domains within the nucleus. These results show that HMGB4 and H1FNT specify distinct chromatin domains at the apical and basal poles of the elongating spermatid nucleus.
Synonyms	dJ1007G16.5
Reference Data
Protein Families	Transcription Factors

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