p53 (TP53) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 500-2000 WB positive control: HT29, HepG2, Hela, 231 cell lysates
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Fusion protein corresponding to a region derived from 1-233 amino acids of human tumor protein p53
Formulation	PBS pH7.3, 0.05% NaN3, 50% glycerol
Concentration	lot specific
Purification	Antigen affinity purification
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	44 kDa
Gene Name	tumor protein p53
Database Link	NP_000537 Entrez Gene 7157 Human UniProt ID P04637
Background	This gene encodes tumor protein p53; which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest; apoptosis; senescence; DNA repair; or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines; where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation; DNA-binding; and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion; and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site; and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies; but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms; which can regulate p53 transcriptional activity.
Synonyms	BCC7; LFS1; P53; TRP53
Reference Data
Protein Families	Druggable Genome, Stem cell - Pluripotency, Transcription Factors
Protein Pathways	Amyotrophic lateral sclerosis (ALS), Apoptosis, Basal cell carcinoma, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Glioma, Huntington's disease, MAPK signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Wnt signaling pathway

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