RNF8 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, WB
Recommend Dilution	RNF8 antibody can be used for detection of RNF8 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunoflourescence starting at 20 µg/mL. For immunofluorescence start at 20 µg/mL. Antibody validated: Western Blot in human samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	RNF8 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human RNF8.
Specificity	At least three isoforms of RNF8 are known to exist; this antibody will detect all three isoforms.
Formulation	RNF8 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1ug/ul
Purification	RNF8 Antibody is affinity chromatography purified via peptide column.
Conjugation	Unconjugated
Storage Condition	Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Gene Name	ring finger protein 8
Database Link	NP_003949 Entrez Gene 58230 MouseEntrez Gene 361815 RatEntrez Gene 9025 Human UniProt ID O76064
Background	RNF8 Antibody: RNF8 was identified as a ubiquitin ligase (E3) containing a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes including UBE2E1/UBCH6, UBE2E2, and UBE2E3. RNF8 assembles at DNA double-strand breaks (DSBs) via interactions though the FHA domain with the adaptor protein MDC1, resulting in an increase in DSB-associated H2A histone ubiquitinations mediated by the associated ubiquitin ligase RNF168 followed by the accumulation of 53BP1 and BRCA1 repair proteins. Together with RNF168, RNF8 plays an integral part of class switch recombination in B cells, allowing the production of several classes of antibodies, through the recruitment of 53BP1 and BRCA1 to the DSB sites.
Synonyms	hRNF8
Reference Data

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