HIF-1 alpha (HIF1A) Mouse Monoclonal Antibody [Clone ID: H1alpha67]

Specifications

Product Data
Clone Name	H1alpha67
Applications	ChIP, ELISA, FC, ICC/IF, IHC, Immunoblotting, IP, WB
Recommend Dilution	Chromatin Immunoprecipitation: 1:10 - 1:500, Western Blot: 1:500 - 1:1000, Flow Cytometry: 1:10 - 1:1000, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry: 1:100 - 1:300, Immunohistochemistry-Paraffin: 1:100 - 1:300, Immunoprecipitation: 1:10, Gel Super Shift Assays: 1:1 - 1:100, Knockout Validated, Chromatin Immunoprecipitation (ChIP): 1:10-1:500, ELISA: 1:100 - 1:2000, Immunoblotting, Knockdown Validated
Reactivity	Bovine, Ferret, Human, Mouse, Porcine, Primate, Rat, Sheep
Host	Mouse
Clonality	Monoclonal
Immunogen	Fusion protein containing amino acids 432-528 of human HIF-1 alpha
Formulation	Tris-glycine, 150mM NaCl pH 7.5 and 0.05% Sodium Azide
Purification	Protein A purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	hypoxia inducible factor 1 alpha subunit
Database Link	NP_001521 Entrez Gene 15251 MouseEntrez Gene 29560 RatEntrez Gene 3091 Human UniProt ID Q16665
Background	Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets.
Synonyms	bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8
Reference Data
Protein Families	Transcription Factors
Protein Pathways	mTOR signaling pathway, Pathways in cancer, Renal cell carcinoma

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