PFKM Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB:1:1000-1:10000
Reactivity	Human, Mouse, Chicken, Dog, Pig, Rat, Xenopus, Bovine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant fragment corresponding to a region within amino acids 1 and 253 of PFK (muscle) (Uniprot ID#P08237)
Formulation	1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Concentration	lot specific
Purification	Purified by antigen-affinity chromatography.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	85 kDa
Gene Name	phosphofructokinase, muscle
Database Link	NP_000280 Entrez Gene 18642 MouseEntrez Gene 65152 RatEntrez Gene 403849 DogEntrez Gene 5213 Human UniProt ID P08237
Background	The PFKM gene encodes the muscle isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. Mammalian PFK is a tetramer made up of various combinations of 3 subunits: muscle (PFKM), liver (PFKL; MIM 171860), and platelet (PFKP; MIM 171840), the genes for which are located on chromosomes 12q13, 21q22, and 10p, respectively. The composition of the tetramers differs according to the tissue type. Muscle and liver PFK are a homotetramers of 4M and 4L subunits, respectively. Erythrocytes contain both L and M subunits, which randomly tetramerize to form M4, L4, and M3L, M2L2, and ML3 hybrid forms of the holoenzyme (Vora et al., 1980 [PubMed 6444721]; Raben and Sherman, 1995 [PubMed 7550225]). [supplied by OMIM]
Synonyms	ATP-PFK; GSD7; PFK-1; PFK1; PFKA; PFKX; PPP1R122
Note	Seq homology of immunogen across species: Chicken (88%), Dog (97%), Pig (95%), Rat (94%), Xenopus laevis (89%), Bovine (96%)
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Fructose and mannose metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway

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