TLR4 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ICC, IF, IHC, WB
Recommend Dilution	TLR4 antibody can be used for detection of TLR4 by Western blot at 2 to 4 µg/mL. Antibody can also be used for immunocytochemistry starting at 2 µg/mL and Immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	TLR4 antibody was raised against a peptide corresponding to 15 amino acids near the amino-terminus of human TLR4.
Formulation	PBS containing 0.02% sodium azide.
Concentration	1ug/ul
Purification	TLR4 Antibody is affinity chromatography purified via peptide column.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	toll like receptor 4
Database Link	NP_612564 Entrez Gene 7099 Human UniProt ID O00206
Background	Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1-3). These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kappaB (4-6). Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis (7).
Synonyms	ARMD10; CD284; TLR-4; TOLL
Reference Data

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