Diablo Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, IP, WB
Recommend Dilution	WB: 1 µg/mL; IF: 10 µg/mL; IHC: 2 µg/mL. Antibody validated: Western Blot in mouse and rat samples; Immunofluorescence and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Smac antibody was raised against a peptide corresponding to amino acids 222 to 237 of murine Smac/DIABLO.
Formulation	PBS containing 0.02% sodium azide.
Concentration	1ug/ul
Purification	Smac Antibody is DEAE purified.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	diablo, IAP-binding mitochondrial protein
Database Link	AF203914 Entrez Gene 66593 Mouse UniProt ID Q9JIQ3
Background	The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO (1,2). Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases (3-6). Smac/DIABLO is expressed in a variety of human and mouse tissues (1,2).
Synonyms	DFNA64; SMAC
Reference Data

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