Dffb Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	WB: 0.5 ug/mL, IHC: 1 ug/mL, IF: 10 ug/mL
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	CAD antibody was raised against a peptide corresponding to amino acids 314 to 329 of murine CAD .
Formulation	PBS containing 0.02% sodium azide.
Purification	Affinity chromatography purified via peptide column
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	DNA fragmentation factor, beta subunit
Database Link	NP_031885 Entrez Gene 13368 Mouse UniProt ID O54788
Background	Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease) (1,2). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40 (3,4). Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively (1, 2, 5). Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei (1-4). Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.
Synonyms	CAD; CPAN; DFF-40; DFF2; DFF40
Reference Data

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