CHFR Goat Polyclonal Antibody

Specifications

Product Data
Applications	IHC
Recommend Dilution	ELISA: 1:64,000. WB: 2.5µg/ml.
Reactivity	Human, Mouse, Rat, Pig, Cow
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence KFNHICEQTRFKN, from the C Terminus of the protein sequence according to NP_060693.
Formulation	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration	lot specific
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase
Database Link	NP_060693 Entrez Gene 231600 MouseEntrez Gene 288734 RatEntrez Gene 55743 Human UniProt ID Q96EP1
Background	E3 ubiquitin-protein ligase required to transiently arrest cells in early prophase when they are exposed to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably promotes the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. This suggests that it may be involved in signaling the presence of mitotic stress caused by microtubule poisons
Synonyms	RNF116; RNF196
Reference Data
Protein Families	Druggable Genome

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