CUL4A Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:1000
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	This Cullin 4A (CUL4A) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-131 amino acids from the N-terminal region of human Cullin 4A (CUL4A).
Formulation	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Concentration	lot specific
Purification	This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	87680 Da
Gene Name	cullin 4A
Database Link	NP_001008895 Entrez Gene 99375 MouseEntrez Gene 8451 Human UniProt ID Q13619
Background	CUL4a is a core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1, DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitiantion of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9.
Synonyms	cullin 4A; OTTHUMP00000018753; OTTHUMP00000018754
Reference Data
Protein Pathways	Nucleotide excision repair, Ubiquitin mediated proteolysis

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.