MAP1LC3A Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ChIP, ELISA, FC, ICC/IF, IHC, Immunoblotting, IP, Simple Western, WB
Recommend Dilution	Chromatin Immunoprecipitation (ChIP), Immunoprecipitation: 20 ug / 500 ug of lysate, Immunohistochemistry: 1:200-1:400, Simple Western: 1:50, Chromatin Immunoprecipitation, Immunocytochemistry/ Immunofluorescence: 1:100-1:300, Immunohistochemistry-Paraffin: 1:200-1:400, Flow Cytometry, ELISA, Immunohistochemistry Whole-Mount, Immunoblotting, Immunohistochemistry-Frozen, Southern Blot, Western Blot: 2.0 ug/ml
Reactivity	Human, Rat, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 25-121).
Formulation	PBS, 0.02% Sodium Azide
Purification	Affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	microtubule associated protein 1 light chain 3 alpha
Database Link	NP_073729 Entrez Gene 64862 RatEntrez Gene 67443 MouseEntrez Gene 84557 Human UniProt ID Q9H492
Background	Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in human. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.
Synonyms	1BLC3; ATG8F; LC3B; MAP1A; MAP1LC3B-a
Reference Data

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