Carbonic Anhydrase IX (CA9) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ChIP, ELISA, FC, ICC/IF, IHC, Immunoblotting, IP, Simple Western, WB
Recommend Dilution	Western Blot: 1 - 3 ug/ml, Proximity Ligation Assay, Gel Super Shift Assays, Simple Western: 1:50, Immunoblotting, ELISA, Immunoprecipitation: 1:10 - 1:500, Microarray, Immunohistochemistry-Paraffin: 1:200 - 1:500, Immunocytochemistry/ Immunofluorescence: 2 - 5 ug/ml, Dual RNAscope ISH-IHC: 1:1000, Chromatin Immunoprecipitation (ChIP): 1:10-1:500, Flow Cytometry: 1:1000, Immunohistochemistry: 1:200 - 1:500, Immunohistochemistry-Frozen: 1:200 - 1:500
Reactivity	Human, Dog
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide derived from a C-terminal sequence of the human CA IX.
Formulation	Tris-citrate/phosphate, pH 7-8, 0.1% sodium Azide
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	carbonic anhydrase 9
Database Link	NP_001207 Entrez Gene 611933 DogEntrez Gene 768 Human UniProt ID Q16790
Background	Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues. Because of its functionality, it has become an important diagnostic marker for various cancers, most notably renal cell carcinoma (RCC). There are many genes that are inducible by hypoxia, via HIF-1 alpha. CA IX is one of the most inducible genes because of its stability and the location of the expressed protein within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia.
Synonyms	CAIX; MN
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Nitrogen metabolism

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